Open the exhaust valve of the horizontal steam sterilizer and let the water boil to remove the cold air from the pot. After the cold air is completely exhausted, close the exhaust valve.

When the pressure in the pot rises to 1Kg/cm2, control the heat source and maintain the pressure for 15 minutes.

Cut off the power, let the temperature in the horizontal steam sterilizer drop naturally, when the pressure drops to 0, open the exhaust valve, loosen the bolt, open the lid, and take out the sterilized items. Otherwise, due to the high pressure in the pot, the pressure difference caused by opening the air valve may cause the medium in the container to splash and cause the cotton plug to contaminate the medium and cause contamination, or even cause the container to explode.

After sterilization, the laboratory utensils are usually dried in an oven at 60°C~80°C to remove the moisture during sterilization and avoid contamination.

1. Items should be placed neatly and stably. and need to leave a gap.

2. When capping, insert the covered exhaust hose into the exhaust slot of the inner sterilization barrel, and tighten the opposite two bolts at the same time in a symmetrical manner, so that the bolts are tight and consistent, and do not leak air .

3. When opening the lid of the horizontal steam sterilizer at the end of sterilization, be careful not to bring your face close, otherwise the steam will cause damage to your face.