Take out the inner bucket of the china vertical steam sterilizer, and then add an appropriate amount of water into the outer pot. When the water level is equal to the triangular shelf, it will be fine. I don’t know if you have noticed this when filling water. It should be noted that neither the mouth end of the Erlenmeyer flask nor the test tube should be in contact with the barrel wall. This is because we do not want the condensed water to wet the wrapping paper and penetrate into the tampon.

Another point, adding a cover, is a common operation when we operate the china vertical steam sterilizer, but we often make mistakes during the operation, we must insert the exhaust hose on the cover into the exhaust of the inner barrel in the slot. Then tighten the two opposite bolts at the same time in a symmetrical manner in pairs, and it must not cause air leakage.

After the required time is completed, be sure to cut off the power or turn off the gas, let the temperature inside the China vertical steam sterilizer drop naturally, at this time, when the pressure on the pressure gauge drops to 0, then open the exhaust valve, loosen the bolts, open the cover, and be careful take out items. Of course, if you find that the pressure gauge has not dropped to 0, we must not open the exhaust valve, because as long as the exhaust valve is opened, the pressure in the pot will suddenly drop, and the culture medium in the container will be rushed out due to the imbalance of internal and external pressure. The mouth of the flask will cause contamination of the tampon with the culture medium, and our operation will end in failure.