The purpose of using pressure steam sterilizer china is to sterilize, so how should we verify the sterilization effect?

Test materials: thermophilic bacillus disc; thermophilic bacillus ATCC7053, spore content 5-105 CFU/sheet; 121°C pressure steam sterilization chemical indicator card; bromocresol purple peptone water culture medium, 116°C for 20 minutes for high-pressure backup Use; 0-150 ℃ leave a thermometer.

Put the thermophilic bacillus disc into the sealed test tube with sterile tweezers, put the chemical indicator card and the thermometer with the left point into the open test tube, prepare 5-10 copies of each of the above two test tubes, and place them in the pressure steamer respectively. At the outlet, the bottom exhaust port and the bottom nozzle or 5 places in the middle of the upper, lower, left, and right sides. If the pressure steamer is on the second floor, it needs to be placed in 10 places. The reserved thermometers can be used for verification tests after being standardized and qualified. The bacterial slices should be placed in the bromocresol purple peptone water medium under strict aseptic conditions, and cultivated at 56-60°C for 24-48 hours, and observe the color change. If the medium turns yellow, it means that the bacteria in the bacterial slices Pyrobacillus has not been completely inactivated, and the bacteria can still grow in the medium, and they decompose glucose to produce acid and turn yellow; if the color of the medium does not change and is still purple, it means that the spores have been inactivated. At the same time, put the untreated paper into the medium as a positive control, and the blank medium without paper as a negative control. The indicator color on the chemical indicator card will change from light yellow to black when it is in a pressure steamer. . With the depth of the color change and comparing it with the control color, it can be judged whether the effect meets the requirements. The chemical indicator card should be stored in a dry place.